| |
| author |
Pooja Kasarapu
| | title |
The effects of clobetasol propionate on neuronal viability and myelination in a cellular model of Alzheimer's disease
| | abstract |
Alzheimer's Disease is a neurodegenerative disorder affecting primarily the elderly
populations and results in gradual memory loss. Research regarding Alzheimer's Disease has
struggled to find cures due to its narrow focus on amyloid beta. Though Alzheimer's Disease is
not classified as a demyelinating disorder, studies have hinted towards the potential role of
myelin loss in Alzheimer's pathology as well as potential ameliorative effects of remyelination
in Alzheimer's models. Clobetasol propionate is a glucocorticoid that has been observed to
increase myelination and differentiation in oligodendrocyte progenitor cells. The purpose of this
study was to understand the effects of clobetasol in neuronal co-cultures to determine whether
clobetasol is toxic towards neurons and to observe whether myelination occurs via any of the
myelinating cells in the co-culture. We conducted experiments to determine ideal and non-toxic
concentrations and exposure periods of clobetasol propionate. These experiments then enabled
further studies in FAB-NMDA models of Alzheimer's Disease in the neuronal culture to
understand whether clobetasol has therapeutic effects in disease conditions. 1 𝜇𝑀 Clobetasol for
48-72 hours were identified as the least toxic conditions towards neurons. Compared to an
untreated cellular model of Alzheimer's, in an Alzheimer's model 1 𝜇𝑀 Clobetasol appeared to
maintain cellular viability and provide slight increases in tubulin stability and myelination. There
was a loss in myelin levels in the Alzheimer's model compared to the control culture with
provides support for the demyelinating hypothesis behind Alzheimer's.
| | school |
The College of Liberal Arts, Drew University
| | degree |
B.A. (2023)
|
| advisor |
Dr. Roger Knowles
|
| full text | PKasarapu.pdf - requires Drew uLogin |
| |
